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. 2003 Jan;185(2):444–452. doi: 10.1128/JB.185.2.444-452.2003

FIG. 4.

FIG. 4.

Northern blot analysis of spoT expression by B. burgdorferi cells in the absence and presence of rabbit serum. Cells samples were prepared and extracted for RNA as described in Materials and Methods. (A) Entire Northern blot of RNA from cells incubated in RPMI (T = 0, 2.5, 5, 10, 15, and 30 min), showing specificity of the DIG-labeled spoT probe, and approximate nucleotide marker. (B) Northern blot showing spoT expression in cells incubated in RPMI (row 1), BSK (row 2), and RPMI plus 6% rabbit serum (row 3) for 0, 2.5, 5, 10, 15, and 30 min. (C) Northern blot showing effect of addition of 6% rabbit serum on expression of spoT (row 1) and ospA (row 2). Cells were starved in RPMI for 15 min (T = −15), and then rabbit serum was added to the culture (T = 0) and samples were taken at 15, 30, 60, and 120 min. (D) Northern blot showing spoT expression in cells incubated in RPMI plus 6% rabbit serum minus methionine (row 1) and RPMI plus 6% rabbit serum minus glucose (row 2). Samples were taken and prepared from cells incubated for 0, 2.5, 5, 10, 15, and 30 min.