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. 2003 Jan;185(2):444–452. doi: 10.1128/JB.185.2.444-452.2003

FIG. 6.

FIG. 6.

Determination of the approximate start and end transcriptional sites of spoT by RT-PCR. RNA was extracted and prepared for RT-PCR from B. burgdorferi cells starved in RPMI for 15 min. RT-PCR amplification was performed using several primer sets, and the reaction products were separated by gel electrophoresis and visualized using ethidium bromide and UV transillumination. Gel lanes: 1, DNA size markers; 2, amplification using primers SpoT F2 and SpoT R1; 3, amplification using primers SpoT F −25 and SpoT R1; 4, amplification using primers SpoT F −50 and SpoT R1; 5, amplification using primers SpoT F2 and SpoT R +800; 6, amplification using primers SpoT F2 and SpoT F2 and Spot R +850; 7, amplification using primers SpoT F2 and SpoT R +950.