TABLE 1.
Primers used in this study
| Primer | Primer sequence (5′ → 3′)a | Coordinates |
|---|---|---|
| SpoT F1 | TTT TTG TCG ACG AGC TC∗C GGT AGT GGA TGA GC | 195219-195233b |
| SpoT F2 | TT GCA CAT TTA ATC AAG ATA AAT G | 195713-195736b |
| SpoT R1 | CGG ATT AGC TGG CAC AT | 196044-196028b |
| SpoT R2 | TTT TTC TGC AG∗G GTT TTG TTC TAA AGA ATC ATC C | 198165-198142b |
| RT F −25 | GAA AAG AGA GCA AGG GCG CTG ATA | 195657-195680b |
| RT F −50 | ACA TTT AAA AAC AAT TTA CGA TCT T | 195632-195656b |
| RT R +800 | TAA GTT TGT TTG TTC TTG TTT GCA T | 196507-196483b |
| RT R +850 | TTT TGT TTT TTG CAA ATT ATT CTT A | 196556-196532b |
| RT R +950 | TTG ATA TTT ATT TTC TTT TGG GCT T | 196656-196632b |
| OspA F1 | GGG AAT AGG TCT AAT ATT AGC CTT | 9411-9434c |
| OspA R1 | CTG CTG ACC CCT CTA ATT TGG TGC C | 10171-10147c |
a
Bold letters indicate recognition sites for the restriction endonucleases used. SpoT F1 includes SacI and SalI sites. SpoT R2 contains a PstI site. ∗ indicates the start of the B. burgdorferi-specific sequence.
b
Coordinates from B. burgdorferi main chromosome.
c
Coordinates from B. burgdorferi linear plasmid 54 (lp54).