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Mobility shift assays of RCT-Cro and wt 434 Cro. (a) Titration of 50 pM 32P-labeled λ OR3 DNA with the indicated concentrations of RCT-Cro. The positions of bound and free DNA are indicated. (b) Competition mobility shift assays with 50 pM 32P-labeled λ OR3 DNA, 0.6 nM RCT-Cro, and the indicated amounts of unlabeled λ OR3 or 434 OR3 DNA.
