TABLE 2.

Functionally important amino acid residues in the E. coli CheW protein compared to aligned sites in the A. brasilense homolog

Residue no.a	Amino acid residue in:		Phenotype or functionb
	E. coli	A. brasilense	Loss of function	CheA binding	Tar binding
33	Ile	Val
36	Val	Val	X		X
38	Glu	Asp	X		X
39	Ile	Val			X
41	Gly	Gly		X	X
45	Val	Ile		X
46	Thr	Thr		X
51	Thr	Ala		X
56	Lys	Alac		X
57	Gly	Gly	X	X
62	Arg	Arg	X
63	Gly	Gly	X		X
65	Ile	Ile		X
86	Thr	Gly	X		X
87	Val	Met			X
88	Val	Ser	X		X
99	Gly	Ser	X		X
105	Val	Val	X		X
108	Val	Val	X		X
128	Gly	Gly
133	Gly	Gly		X	X
156	Met	Met		X
158	Leu	Ala		X

^aE. coli numbering.

^bData are from references 5, 6, and 21.

^cSevere substitution, shown in boldface.