FIG. 2.

Partial organization of the enterocin biosynthetic gene cluster (enc). The orientation of the arrows indicates the direction of each ORF, and those that are shaded are involved in benzoyl-CoA biosynthesis. Suicide plasmids pBM10, pBM12, pBM14, and pBM16 contain internal fragments of encH, encI, encJ, and encN, respectively, and were used for gene inactivation by single recombination. Nc, NcoI. B. subtilis, Bacillus subtilis; R. palustris, Rhodopseudomonas palustris; T. subterraneum, Trifolium subterraneum.