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Southern analysis of NcoI-digested genomic DNA from “S. maritimus” wild type (wt) and mutants KH, KI, KJ, and KN by using the following biotinylated DNA probes: 0.8-kb encH fragment from pBM9 (A); 0.5-kb encI fragment from pBM11 (B); 0.7-kb encJ fragment from pBM13 (C); 0.7-kb encN fragment from pBM15 (D). λ, bacteriophage λ DNA-HindIII digestion marker.
