TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Properties or product | Source or reference |
|---|---|---|
| Strains | ||
| E. coli S17-1 | Contains RP4 integrated into the chromosome | 16 |
| “S. maritimus” BD-26T | Wild type, enterocin+ | 17 |
| “S. maritimus” KP | BD-26T exconjugant containing pBM4, enterocin−; Amr | 20 |
| “S. maritimus” KH | BD-26T exconjugant containing pBM10, enterocin+; Amr | This study |
| “S. maritimus” KI | BD-26T exconjugant containing pBM12, enterocin+; Amr | This study |
| “S. maritimus” KJ | BD-26T exconjugant containing pBM14, enterocin+; Amr | This study |
| “S. maritimus” KN | BD-26T exconjugant containing pBM16, enterocin+; Amr | This study |
| Plasmids | ||
| pCR2.1-TOPO | Vector for cloning PCR products | Invitrogen |
| pKC1139 | E. coli-streptomycete conjugal transfer vector; Amr | 2 |
| pJP15F11 | pOJ446 cosmid clone containing enc gene cluster | 13 |
| pBM9 | 0.8-kb amplified encH internal fragment cloned into pCR2.1-TOPO | This study |
| pBM10 | 0.8-kb EcoRI fragment from pBM9 cloned into pKC1139 | This study |
| pBM11 | 0.5-kb amplified encl internal fragment cloned into pCR2.1-TOPO | This study |
| pBM12 | 0.5-kb EcoRI fragment from pBM11 cloned into pKC1139 | This study |
| pBM13 | 0.7-kb amplified encJ internal fragment cloned into pCR2.1-TOPO | This study |
| pBM14 | 0.7-kb EcoRI fragment from pBM13 cloned into pKC1139 | This study |
| pBM15 | 0.7-kb amplified encN internal fragment cloned into pCR2.1-TOPO | This study |
| pBM16 | 0.7-kb EcoRI fragment from pBM15 cloned into pKC1139 | This study |