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Oligonucleotide primers used to amplify enc fragments for gene disruption experiments
| Targeted gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Product size (bp) |
|---|---|---|---|
| encH | CTGAAGGACTCTCAGGCCG | ATGGGCCTTCCCCGCACCAG | 789 |
| encI | TGACCGTCACGGTCGACAGCG | CTCGACGATGTCGTCGAGGAG | 529 |
| encJ | CGAGTGCCCTGGGCATGCGC | GCTGGGCTGGACCGTGCCGC | 699 |
| encN | CGCAGGGCCGGACGACGGC | GTGCCAGTACTGGGCCACG | 720 |
