TABLE 3.
Characteristics of “S. maritimus” BD-26T-derived enc mutants
| Strain | Gene(s) inactivated | % Productiona
|
% Incorporation of ring-d5-labeled substrates in enterocin
|
|||
|---|---|---|---|---|---|---|
| Cinnamate | Enterocin | Phenylalanine | Benzoate | (3R)-3-Hydroxy-3-phenylpropionate | ||
| Wild type | none | 100 | 100 | 7 | 37 | 3 |
| “S. maritimus” KPb | encP, encO | 0 | 0 | 0 | 100 | Not tested |
| “S. maritimus” KH | encH, encI | 20 | 15 | 9 | 84 | 38 |
| “S. maritimus” KI | encI | 25 | 20 | 9 | 85 | 46c |
| “S. maritimus” KJ | encJ | 160 | 75 | 9 | 43 | 50c |
| “S. maritimus” KN | encN | 100 | 100 | 8 | 0 | 4 |
a
The relative production levels of cinnamic acid and enterocin were compared to those from the wild-type “S. maritimus” strain that were set to 100%. Each percentage is an average value from triplicate experiments with an error of 5 to 10%.
b
Data for mutant KP were previously reported (20) and are provided for comparison.
c
Previously reported in reference 5.