TABLE 1.

SAM synthetase activity and the level of intracellular SAM in S. coelicolor KO-179 and the parental strain 1147^a

Time (h)	SAM synthetase activity (mU/mg of protein)b		SAM level (pmol/mg [wet wt])
	1147	KO-179	1147	KO-179
24	0.91	4.01	ND	ND
36	0.97	10.1	50.7	198.9
48	2.27	15.4	45.5	132.4
60	1.05	10.4	117.7	77.5
72	1.46	6.14	62.0	48.5

^aCells were grown on R5⁻ agar covered with cellophane. Samples were taken at the indicated times, and the SAM synthetase activity and intracellular SAM content were determined as described in Materials and Methods. ND, not determined due to an insufficient amount of cells.

^bOne unit of activity is defined as the amount of enzyme that produced an change in optical density at 340 nm of 12.44/min.