FIG. 4.

Cellulose-binding properties of intact and truncated forms of CelI. Samples of CelI, CelI-t1, and CelI-t2 were subjected to interaction with microcrystalline cellulose and washed, and the retained fraction was released with SDS-containing sample buffer and subjected to SDS-PAGE. Lanes designated (−) represent the samples prior to interaction with cellulose, and those designated (+) represent the sequestered proteins. Only the intact CelI was retained on the cellulose, indicating the requirement of CBM3b for binding.