TABLE 1.
E. coli or serovar Typhimurium strains and plasmids used in this studya
| Strain or plasmid | Laboratory reference | Description | Source or reference |
|---|---|---|---|
| E. coli | |||
| DH5α | NLM264 | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 23 |
| MG1655 | NLM28 | Wild type, derivative of K-12 | Lab collection |
| MG1655 dsbA | NLM1 | MG1655 lacZ::Tn10(TetS) dsbA::kan | Lab collection |
| MC4100 cpxR | NLM367 | MC4100 cpxR::Ω(spec) | 12 |
| Serovar Typhimurium | |||
| SL1344 | NLM2217 | his Strr | 64 |
| SL1344 | NLM2218 | NLM2217 containing pPrdoA | This study |
| SL1344 dsbA | NLM267 | SL1344 dsbA::kan | 59 |
| SL1344 dsbA | NLM2219 | NLM267 containing pPrdoA | This study |
| SL1344 rdoA | NLM2214 | NLM2217 with a rdoA deletion of 766 bp containing pMP190 | This study |
| SL1344 rdoA | NLM2239 | NLM2214 cured of pMP190 | This study |
| SL1344 | NLM2244 | NLM2217 containing pBAD18 | This study |
| SL1344 | NLM2245 | NLM2217 containing pPrdoA, pND18 | This study |
| SL1344 | NLM2246 | NLM2217 containing pPrdoA, pBAD18 | This study |
| SL1344 | NLM2247 | NLM2217 containing pND18, pMEG2 | This study |
| SL1344 | NLM2248 | NLM2217 containing pBAD18, pMEG2 | This study |
| SL1344 rdoA | NLM2253 | NLM2239 containing pPrdoA, pND18 | This study |
| SL1344 rdoA | NLM2254 | NLM2239 pPrdoA, pBAD18 | This study |
| SL1344 rdoA | NLM2255 | NLM2239 containing pND18, pMEG2 | This study |
| SL1344 rdoA | NLM2256 | NLM2239 containing pBAD18, pMEG2 | This study |
| SL1344 rdoA/rdoA+ | NLM2269 | NLM2238 containing pLMN20 | This study |
| SL1344 cpxR | NLM2274 | NLM2217 transduced with cpxR::Ω from NLM367 containing pND18 | This study |
| Plasmids | |||
| pMEG2 | pMP190 with a 258-bp fragment from immediately upstream of serovar Typhimurium dsbA SD sequence | 19 | |
| pPrdoA | pMP190 with a 236-bp fragment from immediately upstream of serovar Typhimurium rdoA SD sequence | This study | |
| pND18 | pBAD18 which contains the E. coli nlpE gene under the control of the PBAD promoter of the araBAD (arabinose) operon | 55 | |
| pMP190 | 15-kb transcriptional fusion vector containing a promoterless lacZ, Cmr | 56 | |
| pMEG1 | pBAD24 with 6.9-kb NcoI fragment from pLAFR2-31 containing dsbA and rdoA | 19 | |
| pLMN20 | pACYC184 containing a 1.5-kb SacII fragment from pMEG1 coding for rdoA | This study |
a
SD, Shine-Dalgarno.