FIG. 1.

Alignment of the amino acid sequences of the ENP1 endopeptidase from B. sphaericus and YcjI (MpaA) from E. coli by T-COFFEE program (http://www.ch.embnet.org/software/TCoffee.html). Identical amino acids and conserved amino acids are indicated by stars and colons, respectively. Amino acid numbers for both proteins are indicated on the right. ENP1 and YcjI sequences are referred to as Q03415 and P51983 in the Swiss-Prot library. The putative zinc binding triad His¹⁶²-Glu¹⁶⁵-His³⁰⁷, catalytic dyad Tyr³⁴⁷-Glu³⁶⁶, and substrate binding residue Asp²⁵⁵ of ENP1 are underlined.