FIG. 1.

Gel mobility shift assay of NAC^WT and NAC^NC at the upstream NAC-binding site from gdhA. Increasing amounts of purified NAC^WT-his (lanes 2 to 7) or NAC^L111K-his (lanes 8 to 13) were incubated with 128 nM DNA fragment containing the upstream NAC-binding site from gdhA. The NAC^WT-his and NAC^L111K-his preparations were found to be 24 and 4.2% active, respectively, in terms of DNA binding (specific activity). Concentrations are reported as the amount of active protein used in the assay. Lanes 1 to 7 contained 0, 25, 50, 75, 100, 125, and 150 nM active NAC^WT-his, respectively. Lanes 8 to 13 contained 25, 50, 75, 100, 125, and 150 nM NAC^L111K-his, respectively.