FIG. 3.

Gel mobility shift assays of NAC^WT or NAC^NC with the full-length gdhA promoter. Increasing amounts of purified NAC^WT-his (lanes 2 to 7) or NAC^L111K-his (lanes 8 to 13) were incubated with 128 nM DNA fragment containing both NAC-binding sites. Lanes 1 to 7 contained 0, 25, 50, 75, 100, 125, and 150 nM active NAC^WT-his, respectively. Lanes 8 to 13 contained 150, 125, 100, 75, 50, and 25 nM NAC^L111K-his, respectively.