FIG. 2.

Inhibition of Guy's 13 binding to solid-phase SA I/II using fluid-phase SA I/II or recombinant fragments of SA I/II. To the wells of an SA I/II-coated ELISA plate were added a range of SA I/II dilutions (a), a range of lysate dilutions (b), or a mixture of two lysates (c and d). In each experiment, a control, containing only PBS (and no inhibitor), was also included and gave an OD₄₅₀ of 0.72 (not shown). The nonrecombinant vector samples represent lysate from IPTG-induced cells harboring nonrecombinant pScFvΔ. Similar results were obtained with lysate from cells harboring the nonrecombinant pEXssΔ3 vector (not shown). Guy's 13 IgG bound to the solid phase was detected by the addition of an HRP-conjugated anti-mouse IgG secondary antibody. Results are presented as the mean OD₄₅₀s for duplicate wells. Lysates of fragments 105, 106, 109, 110, and Pro0-IV were produced using the pScFvΔ expression system, while that of fragment 114 was produced using the pEXssΔ3 vector.