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. 2003 Feb;71(2):754–765. doi: 10.1128/IAI.71.2.754-765.2003

FIG. 4.

FIG. 4.

Binding of recombinant SA I/II fragments to solid-phase SA I/II. Recombinant lysates (at the indicated dilution) were added to the wells of an SA I/II-coated ELISA plate. Following washing, bound recombinant fragments were detected by the addition of either the anti-E tag serum or MOPC 31C as a specificity control. Detection of bound antibody was achieved with an HRP-conjugated secondary antibody. Results are presented as the mean OD450s of duplicate wells. Dilution buffer, DB (see Materials and Methods for composition), was used for dilution of cell lysates and antibodies.