FIG. 5.

Direct ELISA demonstrating the specific interaction between A- and P-region binding sites. ELISA plates coated with recombinant lysate 106 (a) or 109 (b) were incubated with the indicated recombinant lysates (at the indicated dilution), followed by either Guy's 13 or MOPC 31C as a specificity control. Detection of bound antibody was achieved with an HRP-conjugated secondary antibody. Results are presented as the mean OD₄₅₀s of duplicate wells. Dilution buffer, DB (see Materials and Methods for composition), was used for dilution of fluid-phase cell lysates and antibodies.