TABLE 2.
Histological changes in birds following administration of CpG-ODNa
| ODN | Dose (μg/bird) | Route(s) | Gross pathology scoreb on day:
|
||||
|---|---|---|---|---|---|---|---|
| 1 | 3 | 7 | 10 | 14 | |||
| CpG-ODN(2007) | 3.16 | S/C | 3 | 3 | 1 | 1 | 0 |
| 0.1 | S/C | 3 | 0 | 0 | 0 | 0 | |
| 10 | I/M | 3 | 3 | 3 | 3 | 1 | |
| 3.16 | I/M | 1 | 2 | 2 | 0 | 0 | |
| Non-CpG-ODN(2041) | 3.16 | S/C | 2 | 1 | 0 | 0 | 0 |
| 3.16 | I/M | 0 | 0 | 0 | 0 | 0 | |
| Control (saline) | I/M and S/C | 0 | 0 | 0 | 0 | 0 | |
a
Histopathological score (cellular infiltration) following administration of CpG-ODN by either subcutaneous (S/C) or intramuscular (I/M) injection. Cellular infiltration was most pronounced and persisted longest in birds treated with 10 μg of CpG-ODN(2007) by the intramuscular route.
b
0, no visible lesions; 1, focal, mild mononuclear and heterophil infiltrations; 2, locally extensive, moderate mononuclear and heterophil infiltrations; 3, locally extensive, moderate to severe mononuclear and heterophil infiltrations.