TABLE 3.

E. coli septicemia in birds challenged with E. coli following administration of 50 μg of CpG-ODN⁽²⁰⁰⁷⁾ subcutaneously^a

Group	Bird no.	CFU of E. coli/ml on day:
		1	2	4	7	8
Control	1	0	0	0	0	0
	2	2 × 105	Dead
	3	5 × 102	7 × 102	Dead
	4	0	2 × 103	0	0	0
	5	Dead
	6	0	0	0	0	0
	7	1 × 105	Dead
	8	1 × 108	Dead
	9	1 × 104	1 × 104	Dead
	10	1 × 105	1 × 103	Dead
	11	1 × 103	3 × 102	1 × 102	0	0
	12	4 × 105	Dead
	13	1 × 104	Dead
	14	6 × 104	Dead
	15	1 × 105	1 × 105	Dead
	16	1 × 104	2 × 103	Dead
	17	7 × 102	2 × 102	Dead
	18	0	1 × 105	0	0	0
	19	2 × 102	Dead
	20	0	0	0	0	0
Treatment	21	0	0	2 × 104	0	0
	22	0	0	0	0	0
	23	0	0	1 × 103	Dead
	24	0	0	7 × 104	1 × 106	Dead
	25	2 × 102	6 × 102	Dead
	26	0	0	0	0	0
	27	0	0	0	0	0
	28	0	4 × 102	Dead
	29	0	0	0	0	0
	30	0	0	0	0	0
	31	0	6 × 103	Dead
	32	0	0	1 × 102	0	0
	33	0	0	0	0	0
	34	0	0	0	0	0
	35	0	0	0	0	0
	36	0	0	0	0	0
	37	0	0	1 × 102	0	0
	38	0	0	0	0	0
	39	2 × 103	1 × 104	Dead
	40	0	0	0	0	0

^aCFU of E. coli in peripheral blood of birds treated with CpG-ODN⁽²⁰⁰⁷⁾ and a control group on days 1, 2, 4, 7, and 8 following E. coli challenge (n = 20). E. coli was isolated on both sheep blood and MacConkey agar. The majority of birds in the control group had E. coli isolations on days 1 and 2 following E. coli challenge. CpG-ODN⁽²⁰⁰⁷⁾ treatment significantly protected against E. coli infection compared to the level of infection in the control group (P < 0.01).