FIG. 4.

Effects of perfusion on the recovery of L. monocytogenes from A/J mouse tissues. The mice were inoculated i.g. with 10⁶ CFU of L. monocytogenes Scott A. Three days later, some mice were euthanized and their bloodstreams were perfused with saline as described in Materials and Methods. Portions of the brains, spleens, and livers were removed and processed to enumerate viable L. monocytogenes CFU. Other mice were euthanized, and their tissues were removed and processed without perfusion. The results are the means ± SEM. for five mice per group.