FIG. 5.

Anti-M. tuberculosis activity of EPO: halide dependence and requirement for enzymatic activity. A suspension containing 2 × 10⁵ H37Rv cells in 25 mM sodium phosphate buffer (pH 7.0) supplemented with 0.067 M sodium sulfate and (as indicated) 0.1 M NaCl, 0.2 mM NaI, or NaBr either in the presence or in the absence of 0.25 mM H₂O₂ was exposed to 0.2 μM EPO for 60 min of incubation at 37°C. See Materials and Methods for details. Where indicated, aminotriazole (AMT)-treated EPO was used.