FIG. 2.

H. pylori-induced apoptosis in AGS gastric epithelial cells. Subconfluent monolayers of AGS gastric epithelial cells were cultured without or with H. pylori strain 99 (cagA⁺, cytotoxin positive). AGS cells were harvested at 16 h after H. pylori infection and incubated with FITC-conjugated annexin V (AV) and propidium iodide (PI) double staining. Flow cytometric analysis was performed, and the data shown are representative of three separate experiments. The lower right quadrants represent early apoptotic cells that were stained by annexin V but not by propidium iodide. The upper right quadrants represent late apoptotic cells that were stained by both annexin V and propidium iodide.