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cagA+ H. pylori strain-induced Bcl-2 protein expression. AGS gastric epithelial cells were stimulated with H. pylori cagA mutants (strains 28 and 45) and cagA+ strains (strains 92 and 99) at a cell-to-bacterium ratio of 1:100, with or without pretreatment by PD98059 (20 μM). Cell lysates were prepared 16 h after H. pylori infection. Western blot analysis was performed for Bcl-2 family proteins. These experiments were performed on three occasions, and representative blots are shown.
