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Map of the mariner transposon mutagenesis vector pM272B (A) and the M4 procedure (B). (B) Transposon insertion mutants were isolated by a two-step selection procedure, first for Kanr and second for Kanr and Sucr. The numbers in parentheses indicate the frequencies of the occurrence of resistant colonies. Mm, M. marinum; Ec-ori, E. coli origin of replication; WT, wild type; RT, room temperature; sacB−, sacB deficient; mts-ori−, mts-ori deficient.
