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. 2003 Feb;71(2):922–929. doi: 10.1128/IAI.71.2.922-929.2003

FIG. 4.

FIG. 4.

Examination of resistance to light induction of methylene blue (A) and intracellular replication within J774 macrophages (B) for M. marinum pigmentation mutants and the complemented strain. (A) Dilutions of M. marinum strains were plated onto 7H10 plates containing 25 μM methylene blue and incubated in the dark or in ambient light for 7 days. The data are percentages of CFU in the dark divided by CFU in ambient light for each strain. Data for the wild type (WT), three pigmentation mutants, and the complemented MmW04 strain are graphed. The data are means of results from two experiments under the same conditions. (B) CFU associated with J774 cells were enumerated at time zero and after 96 h of intracellular growth. Data for the same strains as those used for panel A are graphed. The means and standard deviations of results from quadruplicate experiments performed on two different days are depicted. pRv2603c-Rv2606c indicates complementation with M. tuberculosis genes encompassing Rv2603c to Rv2606c. Differences between MmW04 and the WT or the complemented strain are statistically significant (P < 0.05); the levels of growth of the WT and the complemented mutant are not different.