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Complementation of carotenoid biosynthesis for M. marinum mutants. (A) A plasmid expressing the M. marinum crtB gene was introduced into both MmW01 (W01) and MmR02 (R02), but it restored pigment synthesis only in MmW01. (B) A plasmid expressing M. tuberculosis genes Rv2603c to Rv2606c was used to complement the MmW04 (W04) mutant to produce wild-type pigment. Both plates were exposed to light for 48 h prior to imaging.
