FIG. 1.
Silica TLC of ASLs from Mycobacterium ulcerans isolates run in chloroform-methanol-water (90:10:1, vol/vol/vol) and visualized by oxidative charring in a ceric molybdate-10% sulfuric acid stain. (A) ASLs extracted from the bacterial CM. (B) ASLs extracted from sterile culture filtrate. MLA/B, mycolactone A/B; Rf, refractive indices; lane M, ASLs extracted from Middlebrook 7H9 media with 10% oleic acid-albumin-dextrose enrichment. Strains are represented in lanes as follows: lane 1, 97-617; lane 2, 97-112; lane 3, 97-101; lane 4, 97-1112; lane 5, 97-110; lane 6, 98-700; lane 7, 98-1137; lane 8, 99-1563; lane 9, 99-1711; lane 10, 97-1116; lane 11, 98-239; lane 12, 97-616; lane 13, 98-156; lane 14, 99-742; lane 15, 97-684; lane 16, 99-1642; lane 17, 99-1722; lane 18, 99-1567; lane 19, 94-1324; lane 20, 94-1325; lane 21, 94-1326; lane 22, 94-1327; lane 23, 94-1328; lane 24, 5114; lane 25, 5143; lane 26, 98-912; lane 27, 8756; lane 28, 1615.