FIG. 2.

YopM localizes to the nucleus in yeast. S. cerevisiae RCD224 was induced for expression of yEGFP-YopM or yEGFP alone by growth in the presence of galactose. (A) The yeast DNA was stained with Hoechst 33342, and blue Hoechst fluorescence is shown in the left panel for the same cells pictured at right, where green yEGFP-YopM fluorescence is shown. Arrows point to nuclei. Note that some cells visible by Hoechst staining (lower left quadrant) are not expressing yEGFP-YopM strongly enough for detection. (B) The localization of green fluorescence is compared for S. cerevisiae RCD224 expressing yEGFP-YopM and yEGFP alone from the vector used to carry the yEGFP-YopM fusion proteins in this study. This and other figures were rendered by using Adobe Photoshop 6.0.