Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1986 Mar;57(3):431–435.

Cellular aspects of the longer-lasting immunity against mouse typhoid infection afforded by the live-cell and ribosomal vaccines.

E Kita, M Emoto, K Yasui, K Yasui, N Katsui, K Nishi, S Kashiba
PMCID: PMC1453836  PMID: 3514442

Abstract

In order to compare the potential of salmonella vaccines prepared from Salmonella typhimurium to provide the longer-lasting protection from the aspects of cell-mediated immunity, groups of mice were immunized with optimal doses of the following preparations: live cells, ribosome-rich extract, acetone-killed cells, and heat-killed cells. At various intervals post-immunization, mouse peritoneal macrophages and splenic T cells were tested for biological activities. The capacity of each vaccine to confer mouse protection against a lethal challenge with S. typhimurium correlated with the degree of macrophage activation engendered by each of them in the early stage of immunization. In the late stage of immunization, the level of mouse protection conferred by each vaccine was found to be based on the capacity of T cells to respond to salmonella antigens, which correlated with the degree of adoptive immunity by T cells. The live-cell and ribosomal vaccines were superior to killed-cell vaccines in inducing the cell-mediated protection. Thus, the longer-lasting immunity provided by the live-cell and ribosomal vaccines can be accounted for by the fact that T cells of mice immunized with both vaccines have the persistent reactivity to salmonella antigens.

Full text

PDF
431

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angerman C. R., Eisenstein T. K. Comparative efficacy and toxicity of a ribosomal vaccine, acetone-killed cells, lipopolysaccharide, and a live cell vaccine prepared from Salmonella typhhimurium. Infect Immun. 1978 Feb;19(2):575–582. doi: 10.1128/iai.19.2.575-582.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Angerman C. R., Eisenstein T. K. Correlation of the duration and magnitude of protection against Salmonella infection afforded by various vaccines with antibody titers. Infect Immun. 1980 Feb;27(2):435–443. doi: 10.1128/iai.27.2.435-443.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins F. M. Effect of adjuvant on immunogenicity of a heat-killed salmonella vaccine. J Infect Dis. 1972 Jul;126(1):69–76. doi: 10.1093/infdis/126.1.69. [DOI] [PubMed] [Google Scholar]
  4. Collins F. M., Mackaness G. B. Delayed hypersensitivity and arthus reactivity in relation to host resistance in salmonella-infected mice. J Immunol. 1968 Nov;101(5):830–845. [PubMed] [Google Scholar]
  5. Germanier R. Immunity in experimental salmonellosis. 3. Comparative immunization with viable and heat-inactivated cells of Salmonella typhimurium. Infect Immun. 1972 May;5(5):792–797. doi: 10.1128/iai.5.5.792-797.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herzberg M., Nash P., Hino S. Degree of immunity induced by killed vaccines to experimental salmonellosis in mice. Infect Immun. 1972 Jan;5(1):83–90. doi: 10.1128/iai.5.1.83-90.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  8. Kenny K., Herzberg M. Early antibody response in mice to either infection or immunization with Salmonella typhimurium. J Bacteriol. 1967 Mar;93(3):773–778. doi: 10.1128/jb.93.3.773-778.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kita E., Kashiba S. Immunogenicity of the ribosomal fraction of Salmonella typhimurium: analysis of humoral immunity. Infect Immun. 1980 Jan;27(1):197–203. doi: 10.1128/iai.27.1.197-203.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kita E., Kashiba S. Immunogenicity of transfer RNA isolated from a two-heptose rough mutant of Salmonella typhimurium LT2 in mouse typhoid infection. Immunology. 1983 Nov;50(3):369–376. [PMC free article] [PubMed] [Google Scholar]
  11. Kita E., Matsuda Y., Matsuda K., Kashiba S. Separate transfer of mouse protection and delayed-type hypersensitivity with Salmonella typhimurium transfer factor. Cell Immunol. 1984 Sep;87(2):528–537. doi: 10.1016/0008-8749(84)90021-2. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Lindberg A. A., Rosenberg L. T., Ljunggren A., Garegg P. J., Svensson S., Wallin N. H. Effect of synthetic disaccharide-protein conjugate as an immunogen in Salmonella infection in mice. Infect Immun. 1974 Sep;10(3):541–545. doi: 10.1128/iai.10.3.541-545.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mackaness G. B., Blanden R. V., Collins F. M. Host-parasite relations in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):573–583. doi: 10.1084/jem.124.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marecki N. M., Hsu H. S., Mayo D. R. Cellular and humoral aspects of host resistance in murine salmonellosis. Br J Exp Pathol. 1975 Jun;56(3):231–243. [PMC free article] [PubMed] [Google Scholar]
  16. Nakoneczna I., Hsu H. S. Histopathological study of protective immunity against murine salmonellosis induced by killed vaccine. Infect Immun. 1983 Jan;39(1):423–430. doi: 10.1128/iai.39.1.423-430.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ornellas E. P., Roantree R. J., Steward J. P. The specificity and importance of humoral antibody in the protection of mice against intraperitoneal challenge with complement-sensitive and complement-resistant Salmonella. J Infect Dis. 1970 Feb;121(2):113–123. doi: 10.1093/infdis/121.2.113. [DOI] [PubMed] [Google Scholar]
  18. Pick E., Keisari Y. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods. 1980;38(1-2):161–170. doi: 10.1016/0022-1759(80)90340-3. [DOI] [PubMed] [Google Scholar]
  19. Svenson S. B., Nurminen M., Lindberg A. A. Artificial Salmonella vaccines: O-antigenic oligosaccharide-protein conjugates induce protection against infection with Salmonella typhimurium. Infect Immun. 1979 Sep;25(3):863–872. doi: 10.1128/iai.25.3.863-872.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES