TABLE 6.
Inflammatory cells present in BAL fluid of B10 mice with C2D whole bone marrow or C2D macrophage adoptive transfer and P. pneumotropica challenge
Time (h) postinfectiona | No. of inflammatory cells present (mean ± SEM) afterb:
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Whole bone marrow transfer
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C2D macrophage transfer
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Macrophage
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Neutrophil
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Macrophage
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Neutrophil
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No transfer | Transferc | No transfer | Transfer | No transfer | Transfer | No transfer | Transfer | |
0 | 94 ± 2 | 97 ± 1 | 2 ± 1 | 1 ± 0 | 98 ± 3 | 98 ± 2 | 2 ± 1 | 1 ± 0 |
24 | 56 ± 2 | 64 ± 2 | 32 ± 1 | 29 ± 1 | 58 ± 2 | 72 ± 2 | 30 ± 2 | 19 ± 1 |
48 | 50 ± 2 | 54 ± 1 | 36 ± 2 | 34 ± 2 | 50 ± 4 | 54 ± 2 | 36 ± 3 | 34 ± 2 |
72 | 44 ± 3 | 53 ± 2 | 49 ± 3 | 41 ± 1 | 41 ± 4 | 50 ± 2 | 49 ± 4 | 37 ± 1 |
120 | 56 ± 2 | 59 ± 2 | 33 ± 2 | 31 ± 2 | 57 ± 2 | 61 ± 2 | 32 ± 2 | 28 ± 3 |
240 | 67 ± 3 | 68 ± 2 | 26 ± 3 | 22 ± 2 | 78 ± 4 | 84 ± 1 | 17 ± 4 | 10 ± 2 |
BAL fluid was collected from six animals per treatment group at 24, 48, 72, 120, 240 h post-bacterial challenge.
Values based on six mice per treatment group. Percentage of cells determined by scoring ≥200 cells per mouse sample. Significant differences (P < 0.05) between transfer and no transfer at 0, 24, and 72 has determined by χ2.
Recipient animals received injections of 2.0 × 107 cells per mouse i.p.