FIG. 4.
FACS analysis showing phagocytosis of necrotic and apoptotic Jurkat cells by NH4Cl-treated E. histolytica trophozoites. Amebic phagocytosis of CFSE-labeled necrotic (green) and TAMRA-labeled apoptotic (red) Jurkat cells (E. histolytica-necrotic Jurkat-apoptotic Jurkat cell ratio, 1:1:1; 37°C, 10 min) was measured by flow cytometry. A representative two-color dot plot showing amebic fluorescence following incubation with both labeled Jurkat cell populations is shown. Values are percentages of amebae positive for phagocytosis of apoptotic (upper left), necrotic (lower right), or both apoptotic and necrotic (upper right) cells (mean and SD, n = 3). The asterisk indicates a P value of <0.001 for a comparison with ingestion of necrotic cells.