FIG. 1.
Schematic representation of the promoter-trapping vector used in the present study. pXXMCS-5 is a versatile plasmid conveniently allowing cloning of either promoter cassettes or reporter genes. This plasmid was used for (i) evaluation of various promoters and reporter genes, (ii) construction and screening of a genomic B. anthracis library, and (iii) expression of the pagA gene. Restriction sites for insertion of the various promoters and different reporter genes, as well as the respective promoter cassettes, are indicated. The linker DNA sequence between the promoter and the reporter gene insertion sites contains the RBS.