TABLE 1.
Plasmids and strains
| Plasmid or strain | Relevant characteristics | Source or reference |
|---|---|---|
| Plasmids | ||
| pUB110 | Kmr in bacilli; Staphylococcus aureus origin of replication | Sigma |
| pGEM-3Z | Apr in E. coli | Promega |
| pGFP | Source of wt gfp reporter gene | Clontech |
| pGFPuv | Source of gfpuv reporter gene | Clontech |
| pEGFP | Source of egfp reporter gene | Clontech |
| pXXMCS-5 | E. coli-Bacillus shuttle vector; composed of an altered fusion of pGEM-3Z/pUB110 without promoter or reporter gene; Apr Kmr | Laboratory stock |
| pGwtMCS-5 | Promoterless wt gfp gene from pGFPwt; inserted in pXXMCS-5 | This work |
| pGuvMCS-5 | Promoterless gfpuv gene from pGFPuv; inserted in pXXMCS-5 | This work |
| pGeMCS-5 | Promoterless egfp gene from pEGFP; inserted in pXXMCS-5 | This work |
| pPA20-α | Source for Pamy promoter and pagA gene | 11 |
| pGwtamy | Pamy promoter fused to wt gfp gene cloned in pXXMCS-5 | This work |
| pGwttms | Ptms promoter fused to wt gfp gene cloned in pXXMCS-5 | This work |
| pGwt43 | P43 promoter region fused to wt gfp gene cloned in pXXMCS-5 | This work |
| pGuvamy | Pamy promoter fused to gfpuv gene cloned in pXXMCS-5 | This work |
| pGuv43 | P43 promoter region fused to gfpuv gene cloned in pXXMCS-5 | This work |
| pGuvsapS | Psapshort promoter region fused to gfpuv gene cloned in pXXMCS-5 | This work |
| pGuvsapL | Psaplong promoter region fused to gfpuv gene cloned in pXXMCS-5 | This work |
| pGe43 | P43 promoter region fused to egfp gene cloned in pXXMCS-5 | This work |
| pAMCS-5 | Promoterless pagA gene from pPA20-α; inserted in pXXMCS-5 | This work |
| pApag | Formerly named pAαUB; Ppag promoter region fused to pagA gene cloned in pGEM-3Z/pUB110 fusion vector; Apr Kmr | 11 |
| pAamy | Formerly named pαAUB; Pamy promoter fused to pagA gene cloned in pGEM-3Z/pUB110 fusion vector; Apr Kmr | 11 |
| pA43 | P43 promoter region fused to pagA gene cloned in pXXMCS-5 | This work |
| pAsapS | Psapshort promoter region fused to pagA gene cloned in pXXMCS-5 | This work |
| pAsapL | Psaplong promoter region fused to pagA gene cloned in pXXMCS-5 | This work |
| pAntr | Pntr promoter region of library clone L-19 fused to pagA gene cloned in pXXMCS-5 | This work |
| B. anthracis Δ14185 | pXO1− pXO2− strain; a derivative of ATCC 14185 | 11 |
| B. subtilis WB600 | ΔnprE ΔaprE Δepr Δbpf Δmpr ΔnprB | 48 |
| E. coli | ||
| DH5α | recA1 | Clontech |
| GM2929 | dam13::Tn9 (Cmr) dcm-6 | New England Biolabs |