. 2003 Feb;71(2):801–813. doi: 10.1128/IAI.71.2.801-813.2003

TABLE 3.

Immunization of guinea pigs with B. anthracis strains harboring various promoter-pagA cassettes

Plasmid^a	In vitro rPA production (μg/ml)^b	ELISA titer^c
Plasmid^a	In vitro rPA production (μg/ml)^b	Anti-PA	Anti-core
pA_pag	19 ± 3	15 ± 2	190 ± 5
pA_amy	120 ± 10	950 ± 5	190 ± 5
pA_sapS	130 ± 30	250 ± 5	200 ± 5
pA_sapL	130 ± 40	100 ± 5	540 ± 10
Saline	0	<10	<10

Guinea pigs were inoculated subcutaneously with a single dose of 10⁶ spores of B. anthracis recombinant strains carrying the indicated plasmids.

Mean values (determined by ELISA of PA in culture supernatant at an A₅₅₀ of 7 to 8) ± the standard error of five experiments.

Values represent antibody titers (geometric mean titers) ± the standard deviation from 10 animals determined 6 weeks postinoculation of spores.