FIG. 2.

Induction of ceramide by SMase in HDMEC. HDMEC were incubated with SMase (0.067 U/ml) for shorter (A) or longer (B) periods of time, and cellular ceramide was quantified as described in Materials and Methods. The data are representative of results from four different experiments performed under these conditions. C₁₈ ceramide was undetectable at zero time. C, untreated control cells; C-18 CER, C-₁₈ ceramide.