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Effect of SMase on induction of the Gb3 biosynthetic pathway enzymes Gb3 synthase (A) and CGT (B) in HDMEC. HDMEC were incubated with SMase (0.067 U/ml) for the times indicated, the total RNA was isolated, and Gb3 synthase mRNA (A) or CGT mRNA (B) was measured as described in Materials and Methods. The data shown are representative of results from triplicate experiments. The intensities of the mRNA bands were normalized to that of the 18S RNA loading. Control, without SMase treatment.
