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. 2000 Dec 26;98(1):48–53. doi: 10.1073/pnas.240458597

Figure 1.

Figure 1

Induction of Reg gene by IL-6/dexamethasone/PARP inhibitors. RINm5F β cells were treated with IL-1β, TNFα, IFNγ, IL-6, dexamethasone (Dx), nicotinamide (NA), 3-aminobenzamide (3AB), Reg protein, and anti-rat Reg monoclonal antibody (17) (αReg). Values represent mean ± SEM of at least seven experiments. Statistical analyses were performed using Student's t test. (A) RT-PCR analysis. Expression of Reg and of the control glyceraldehyde-3-phosphate dehydrogenase (G3PDH) gene were analyzed by RT-PCR. (B) WST-1 assay. Reg (1.5 μg/ml) was added to the medium. WST-1 cleavages were significantly increased by the incubation with IL-6/Dx, IL-6/Dx/NA, IL-6/Dx/3AB, and Reg (P < 0.001). (C) BrdUrd incorporation. Reg (1.5 μg/ml) and αReg (15 μg/ml) were added to the medium. (D) Immunoblot analysis. Secreted Reg protein was analyzed by immunoblot of the culture medium of the RINm5F cells.