Fig. 7. Inhibition of in vitro ubiquitylation of the Runt domain by PEBP2β. Purified His₆-tagged PEBP2β (β) or the Runt domain (RD) were ubiquitylated in vitro. In the case of lanes 10–12, the Runt domain was incubated with increasing amounts of PEBP2β (at ratios of 1:1, 1:5, 1:10) 2 h before ubiquitylation. After ubiquitylation was stopped, western blotting was performed with anti-αB1 and anti-β2.