Skip to main content
. 2001 Jan 2;98(1):54–59. doi: 10.1073/pnas.98.1.54

Figure 1.

Figure 1

Influence of the presence of a 5′ tail on the helicase activity of Mcm4/6/7 complex. DNA helicase activity assays were carried out with increasing amounts of Mcm4/6/7 protein and 5 fmol of the indicated substrates with (B) or without (A) a 40-nt oligo(dT) tail at its 5′ end, as described in Materials and Methods. Lane B, boiled substrate; lane 1, no Mcm4/6/7 protein was added; lanes 2–5 contained 25, 50, 100, or 200 ng of the Mcm4/6/7 complex, respectively.