Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1983 Jul;49(3):431–438.

Induction of syngeneic tumour-specific immunity by liposomes reconstituted with L2C tumour-cell antigens.

A J Schroit, M E Key
PMCID: PMC1454301  PMID: 6862519

Abstract

Liposomes composed of phosphatidylcholine and phosphatidylserine were reconstituted by detergent dialysis with 3 M K Cl extracts of L2C tumour cells. Liposomes containing L2C antigens were as antigenic as intact tumour cells in the elicitation of delayed-type hypersensitivity responses in strain-2 guinea-pigs previously immunized against L2C tumours. Soluble L2C antigens were devoid of immunoprotective activity, whereas the reconstituted liposomes were capable of protecting animals against up to approximately 100 times the minimal lethal dose of tumour cells. Moreover, the reconstituted liposomes were as antigenic and immunoprotective as viable (irradiated) cells.

Full text

PDF
433

Images in this article

434Figure 2
on p.434

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azuma I., Sugimura K., Yamawaki M., Uemiya M., Kusumoto S., Okada S., Shiba T., Yamamura Y. Adjuvant activity of synthetic 6-O-"mycoloyl"-N-acetylmuramyl-L-alanyl-D-isoglutamine and related compounds. Infect Immun. 1978 Jun;20(3):600–607. doi: 10.1128/iai.20.3.600-607.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun D. P., Hengst J. C., Mokyr M. B., Dray S. Antitumor immunity in strain 2 guinea pigs immunized with potassium chloride extracts of L2C tumor cells. J Natl Cancer Inst. 1978 Apr;60(4):899–903. doi: 10.1093/jnci/60.4.899. [DOI] [PubMed] [Google Scholar]
  3. CONGDON C. C., LORENZ E. Leukemia in guinea-pigs. Am J Pathol. 1954 Mar-Apr;30(2):337–359. [PMC free article] [PubMed] [Google Scholar]
  4. Engelhard V. H., Guild B. C., Helenius A., Terhorst C., Strominger J. L. Reconstitution of purified detergent-soluble HLA-A and HLA-B antigens into phospholipid vesicles. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3230–3234. doi: 10.1073/pnas.75.7.3230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerlier D., Sakai F., Doré J. F. Induction of antibody response to liposome-associated Gross-virus cell-surface antigen (GCSAa). Br J Cancer. 1980 Feb;41(2):236–242. doi: 10.1038/bjc.1980.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hollander N., Mehdi S. Q., Weissman I. L., McConnell H. M., Kriss J. P. Allogeneic cytolysis of reconstituted membrane vesicles. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4042–4045. doi: 10.1073/pnas.76.8.4042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hu C. P., Konen T. G., Hoes C., Green I. Solubilization and partial characterization of a tumor-associated transplantation antigen of the guinea pig L2C leukemia. J Immunol. 1978 May;120(5):1521–1525. [PubMed] [Google Scholar]
  8. Kotani S., Kinoshita F., Morisaki I., Shimono T., Okunaga T., Takada H., Tsujimoto M., Watanabe Y., Kato K., Shiba T. Immunoadjuvant activities of synthetic 6-O-acyl-N-acetylmuramyl-L-alanyl-D-isoglutamine with special reference to the effect of its administration with liposomes. Biken J. 1977 Dec;20(3-4):95–103. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Littman D. R., Cullen S. E., Schwartz B. D. Insertion of Ia and H-2 alloantigens into model membranes. Proc Natl Acad Sci U S A. 1979 Feb;76(2):902–906. doi: 10.1073/pnas.76.2.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pellis N. R., Kahan B. D. Specific tumor immunity induced with soluble materials: restricted range of antigen dose and of challenge tumor load for immunoprotection. J Immunol. 1975 Dec;115(6):1717–1722. [PubMed] [Google Scholar]
  12. Raphael L., Tom B. H. In vitro induction of primary and secondary xenoimmune responses by liposomes containing human colon tumor cell antigens. Cell Immunol. 1982 Aug;71(2):224–240. doi: 10.1016/0008-8749(82)90258-1. [DOI] [PubMed] [Google Scholar]
  13. Reisfeld R. A., Pellegrino M. A., Kahan B. D. Salt extraction of soluble HL-A antigens. Science. 1971 Jun 11;172(3988):1134–1136. doi: 10.1126/science.172.3988.1134. [DOI] [PubMed] [Google Scholar]
  14. Sakai F., Gerlier D., Doré J. F. Association of gross virus-associated cell-surface antigen with liposomes. Br J Cancer. 1980 Feb;41(2):227–235. doi: 10.1038/bjc.1980.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sherman L., Burakoff S. J., Mescher M. F. Induction of allogeneic cytolytic T lymphocytes by partially purified membrane glycoproteins. Cell Immunol. 1980 Apr;51(1):141–150. doi: 10.1016/0008-8749(80)90244-0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES