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Fig. 8. Binding studies on APCΔdoc1. Native gels were used to analyse protein–protein interactions between (A) [35S]His6-Doc1p and APC or APCΔdoc1; (B) [35S]His6-Cdh1p and APC or APCΔdoc1, in the presence or absence of Doc1p purified from E.coli; (C) [35S]His6-Cdc20p and APC or APCΔdoc1; (D) [35S]Clb2p or [35S]His6-Hsl1667–872 and APC or APCΔdoc1, in the presence or absence of Doc1p purified from E.coli and His6-Cdh1p. APCΔdoc1 sometimes runs slightly faster than APC (C).
