Figure 2.

Subcellular localization and colocalization of individual degradosome proteins by using immunogold double labeling. Ultrathin sections of E. coli cells singly labeled with anti-RNase E (A) or anti-β-Gal control antibody (B). (C) Double labeling of cells with anti-RNase E (15 nm gold-IgG complexes) and anti-RhlB helicase (5 nm gold-IgG complexes). (D and E) The 5 nm gold-IgG complexes labeled PNPase and enolase, respectively, are shown, whereas labeling of RNase E was the same as in C. Log phase cultured cells were used for the studies. (Bars = 100 nm.)