Figure 4.

Effect of RNase E truncation on localization of degradosome proteins. Subcellular localization of RhlB helicase and RNase E in mutant BZ99, which produces an Rne protein lacking the C-terminal region (A), or in BZ99 cells that also contain the pRne plasmid, which encodes the full length Rne protein (B). Ultrathin sections of E. coli cell labeled with anti-RNase E antibody are shown in Top. Cells labeled with anti-RhlB helicase antibody are shown in Bottom. Log phase cultured cells were used for the studies. (Bars = 100 nm.)