Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1984 Jul;52(3):501–509.

Functional properties of lymphocytes isolated from murine small intestinal epithelium.

S B Dillon, T T MacDonald
PMCID: PMC1454501  PMID: 6611297

Abstract

Intraepithelial lymphocytes (IEL) were isolated from murine small intestine using a modification of previously published procedures. Analysis of IEL by immunofluorescence using monoclonal antibodies showed they were predominantly Lyt-2+ cells, with relatively few B cells or macrophages present. IEL cultured at sufficiently high cell densities proliferated in response to concanavalin A (Con A), phytohaemagglutin (PHA) and lipopolysaccharide (LPS). IEL were also capable of recognizing alloantigens in an in vivo graft-versus-host assay and in an in vitro mixed lymphocyte reaction. These studies therefore confirm that murine IEL contain cells with immunologic properties characteristic of typical T lymphocytes.

Full text

PDF
501

Images in this article

504Figure 1
on p.504

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnaud-Battandier F., Bundy B. M., O'Neill M., Bienenstock J., Nelson D. L. Cytotoxic activities of gut mucosal lymphoid cells in guinea pigs. J Immunol. 1978 Sep;121(3):1059–1065. [PubMed] [Google Scholar]
  2. Cerf-Bensussan N., Schneeberger E. E., Bhan A. K. Immunohistologic and immunoelectron microscopic characterization of the mucosal lymphocytes of human small intestine by the use of monoclonal antibodies. J Immunol. 1983 Jun;130(6):2615–2622. [PubMed] [Google Scholar]
  3. Crabbé P. A., Nash D. R., Bazin H., Eyssen H., Heremans J. F. Immunohistochemical observations on lymphoid tissues from conventional and germ-free mice. Lab Invest. 1970 May;22(5):448–457. [PubMed] [Google Scholar]
  4. Davies M. D., Parrott D. M. Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine. Gut. 1981 Jun;22(6):481–488. doi: 10.1136/gut.22.6.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ferguson A. Intraepithelial lymphocytes of the small intestine. Gut. 1977 Nov;18(11):921–937. doi: 10.1136/gut.18.11.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ferguson A., Parrott D. M. The effect of antigen deprivation on thymus-dependent and thymus-independent lymphocytes in the small intestine of the mouse. Clin Exp Immunol. 1972 Dec;12(4):477–488. [PMC free article] [PubMed] [Google Scholar]
  7. Greenwood J. H., Austin L. L., Dobbins W. O., 3rd In vitro characterization of human intestinal intraepithelial lymphocytes. Gastroenterology. 1983 Nov;85(5):1023–1035. [PubMed] [Google Scholar]
  8. Guy-Grand D., Griscelli C., Vassalli P. The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978 Dec 1;148(6):1661–1677. doi: 10.1084/jem.148.6.1661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lyscom N., Brueton M. J. Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies. Immunology. 1982 Apr;45(4):775–783. [PMC free article] [PubMed] [Google Scholar]
  10. MacDonald T. T., Carter P. B. Isolation and functional characteristics of adherent phagocytic cells from mouse Peyer's patches. Immunology. 1982 Apr;45(4):769–774. [PMC free article] [PubMed] [Google Scholar]
  11. Mayrhofer G., Whately R. J. Granular intraepithelial lymphocytes of the rat small intestine. I. Isolation, presence in T lymphocyte-deficient rats and bone marrow origin. Int Arch Allergy Appl Immunol. 1983;71(4):317–327. doi: 10.1159/000233414. [DOI] [PubMed] [Google Scholar]
  12. Parrott D. M., Tait C., MacKenzie S., Mowat A. M., Davies M. D., Micklem H. S. Analysis of the effector functions of different populations of mucosal lymphocytes. Ann N Y Acad Sci. 1983 Jun 30;409:307–320. doi: 10.1111/j.1749-6632.1983.tb26879.x. [DOI] [PubMed] [Google Scholar]
  13. Reynolds J. D., Morris B. The influence of gut function on lymphoid cell populations in the intestinal mucosa of lambs. Immunology. 1983 Jul;49(3):501–509. [PMC free article] [PubMed] [Google Scholar]
  14. Röpke C., Everett N. B. Kinetics of intraepithelial lymphocytes in the small intestine of thymus-deprived mice and antigen-deprived mice. Anat Rec. 1976 May;185(1):101–108. doi: 10.1002/ar.1091850110. [DOI] [PubMed] [Google Scholar]
  15. Schrader J. W., Scollay R., Battye F. Intramucosal lymphocytes of the gut: Lyt-2 and thy-1 phenotype of the granulated cells and evidence for the presence of both T cells and mast cell precursors. J Immunol. 1983 Feb;130(2):558–564. [PubMed] [Google Scholar]
  16. Selby W. S., Janossy G., Goldstein G., Jewell D. P. T lymphocyte subsets in human intestinal mucosa: the distribution and relationship to MHC-derived antigens. Clin Exp Immunol. 1981 Jun;44(3):453–458. [PMC free article] [PubMed] [Google Scholar]
  17. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
  18. Tadakuma T., Kühner A. L., Rich R. R., David J. R., Pierce C. W. Biological expressions of lymphocyte activation. V. Characterization of a soluble immune response suppressor (SIRS) produced by concanavalin A-activated spleen cells. J Immunol. 1976 Jul;117(1):323–330. [PubMed] [Google Scholar]
  19. Tagliabue A., Befus A. D., Clark D. A., Bienenstock J. Characteristics of natural killer cells in the murine intestinal epithelium and lamina propria. J Exp Med. 1982 Jun 1;155(6):1785–1796. doi: 10.1084/jem.155.6.1785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tseng J. Expression of immunoglobulin isotypes by lymphoid cells of mouse intestinal lamina propria. Cell Immunol. 1982 Nov 1;73(2):324–336. doi: 10.1016/0008-8749(82)90459-2. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES