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Fig. 2. LC/MS analysis. Mass chromatogram (m/z 973.5) of the peptide mixture generated by in-gel digestion of native Gαs with Lys-C (A) and of the peptide palm-GCLGNSK prepared by solid-phase peptide synthesis (B). Samples were separated by HPLC and detected on-line by electrospray mass spectrometry. Indicated are signal intensities in arbitrary units (u).
