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Glutamic semialdehyde in HeLa cells subjected to oxidative stress by exposure to glucose oxidase/glucose. Cells were grown to confluency and then glucose oxidase/glucose added to the medium to generate the indicated concentration of H2O2 as determined analytically. Results are expressed as mmol/mol of an average 50-kDa protein. Each bar represents analysis of three independent protein samples from a single culture. Error bars are SD.
