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. 1988 Jan;63(1):145–150.

Cloned bovine cytolytic T cells recognize bovine herpes virus-1 in a genetically restricted, antigen-specific manner.

G A Splitter 1, L Eskra 1, A F Abruzzini 1
PMCID: PMC1454703  PMID: 2448231

Abstract

The inability to demonstrate bovine herpes virus-1 (BHV-1)-specific lymphocyte responses from BHV-1-infected cattle has been a major difficulty in confirming the importance of cellular effector mechanisms during BHV-1 infection. We have examined the capacity of bovine cytolytic T-cell clones to lyse BHV-1-infected, concanavalin A-stimulated blast cells. Cytolysis was as high as 58% at an effector to target (E:T) ratio of 1:1. All cytolytic T-cell clones produced were genetically restricted in killing cells of the autologous genotype. Cytotoxic T-lymphocyte (CTL) clones were specific for BHV-1 but not related herpes viruses, i.e. BHV-2 and pseudorabies virus. These results provide evidence that cytolytic T lymphocytes have an antigen-specific role in the immune response of cattle against BHV-1, and that CTL may serve as effector cells in the identification of glycoproteins useful in recombinant vaccine preparation since only determinants of BHV-1 were recognized.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun R. W., Teute H. K., Kirchner H., Munk K. Replication of herpes simplex virus in human T lymphocytes: characterization of the viral target cell. J Immunol. 1984 Feb;132(2):914–919. [PubMed] [Google Scholar]
  2. Brigham S. H., Rossi C. R. Cell-mediated cytotoxicity of peripheral blood mononuclear cells stimulated in vitro for infectious bovine rhinotracheitis virus-infected cells. Vet Immunol Immunopathol. 1986 Nov;13(3):203–212. doi: 10.1016/0165-2427(86)90073-5. [DOI] [PubMed] [Google Scholar]
  3. Carter V. C., Schaffer P. A., Tevethia S. S. The involvement of herpes simplex virus type 1 glycoproteins in cell-mediated immunity. J Immunol. 1981 May;126(5):1655–1660. [PubMed] [Google Scholar]
  4. Hyland S. J., Easterday B. C., Pawlisch R. Antibody levels and immunity to infectious bovine rhinotracheitis virus (IBR) infections in Wisconsin dairy cattle. Dev Biol Stand. 1975;28:510–525. [PubMed] [Google Scholar]
  5. Killington R. A., Yeo J., Honess R., Watson D. H., Duncan B. E., Halliburton I. W., Mumford J. Comparative analyses of the proteins and antigens of five herpesviruses. J Gen Virol. 1977 Nov;37(2):297–310. doi: 10.1099/0022-1317-37-2-297. [DOI] [PubMed] [Google Scholar]
  6. Larsen H. S., Russell R. G., Rouse B. T. Recovery from lethal herpes simplex virus type 1 infection is mediated by cytotoxic T lymphocytes. Infect Immun. 1983 Jul;41(1):197–204. doi: 10.1128/iai.41.1.197-204.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marshall R. L., Rodriguez L. L., Letchworth G. J., 3rd Characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods. J Virol. 1986 Mar;57(3):745–753. doi: 10.1128/jvi.57.3.745-753.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller-Edge M., Splitter G. A. Bovine interleukin 2 (IL-2) production and activity on bovine and murine cell lines. Vet Immunol Immunopathol. 1984 Sep;7(2):119–130. doi: 10.1016/0165-2427(84)90013-8. [DOI] [PubMed] [Google Scholar]
  9. Miller-Edge M., Splitter G. Detection of impaired T cell-mediated immune responses to herpesvirus (BHV-1) in cattle. Vet Immunol Immunopathol. 1986 Sep;13(1-2):1–18. doi: 10.1016/0165-2427(86)90044-9. [DOI] [PubMed] [Google Scholar]
  10. Miller-Edge M., Splitter G. Patterns of bovine T cell-mediated immune responses to bovine herpesvirus 1. Vet Immunol Immunopathol. 1986 Dec;13(4):301–319. doi: 10.1016/0165-2427(86)90024-3. [DOI] [PubMed] [Google Scholar]
  11. Nahmias A. J., Roizman B. Infection with herpes-simplex viruses 1 and 2. 3. N Engl J Med. 1973 Oct 11;289(15):781–789. doi: 10.1056/NEJM197310112891505. [DOI] [PubMed] [Google Scholar]
  12. Nash A. A., Phelan J., Wildy P. Cell-mediated immunity in herpes simplex virus-infected mice: H-2 mapping of the delayed-type hypersensitivity response and the antiviral T cell response. J Immunol. 1981 Apr;126(4):1260–1262. [PubMed] [Google Scholar]
  13. Oakes J. E. Role for cell-mediated immunity in the resistance of mice to subcutaneous herpes simplex virus infection. Infect Immun. 1975 Jul;12(1):166–172. doi: 10.1128/iai.12.1.166-172.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Osorio F. A., Reed D. E., Van der Maaten M. J., Metz C. A. Comparison of the herpesviruses of cattle by DNA restriction endonuclease analysis and serologic analysis. Am J Vet Res. 1985 Oct;46(10):2104–2109. [PubMed] [Google Scholar]
  15. Pfizenmaier K., Jung H., Starzinski-Powitz A., Röllinghoff M., Wagner H. The role of T cells in anti-herpes simplex virus immunity. I. Induction of antigen-specific cytotoxic T lymphocytes. J Immunol. 1977 Sep;119(3):939–944. [PubMed] [Google Scholar]
  16. Rouse B. T., Babiuk L. A. The direct antiviral cytotoxicity by bovine lymphocytes is not restricted by genetic incompatibility of lymphocytes and target cells. J Immunol. 1977 Feb;118(2):618–624. [PubMed] [Google Scholar]
  17. Rouse B. T., Horohov D. W. Cytotoxic T lymphocytes in herpesvirus infections. Vet Immunol Immunopathol. 1984 May;6(1-2):35–66. doi: 10.1016/0165-2427(84)90048-5. [DOI] [PubMed] [Google Scholar]
  18. Sethi K. K., Omata Y., Schneweis K. E. Protection of mice from fatal herpes simplex virus type 1 infection by adoptive transfer of cloned virus-specific and H-2-restricted cytotoxic T lymphocytes. J Gen Virol. 1983 Feb;64(Pt 2):443–447. doi: 10.1099/0022-1317-64-2-443. [DOI] [PubMed] [Google Scholar]
  19. Sethi K. K., Stroehmann I., Brandis H. Human T-cell cultures from virus-sensitized donors can mediate virus-specific and HLA-restricted cell lysis. Nature. 1980 Aug 14;286(5774):718–720. doi: 10.1038/286718a0. [DOI] [PubMed] [Google Scholar]
  20. Snowden B. W., Kinchington P. R., Powell K. L., Halliburton I. W. Antigenic and biochemical analysis of gB of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1. J Gen Virol. 1985 Feb;66(Pt 2):231–247. doi: 10.1099/0022-1317-66-2-231. [DOI] [PubMed] [Google Scholar]
  21. Splitter G. A., Eskra L. Bovine T lymphocyte response to bovine herpesvirus-1: cell phenotypes, viral recognition and acid-labile interferon production. Vet Immunol Immunopathol. 1986 Mar;11(3):235–250. doi: 10.1016/0165-2427(86)90004-8. [DOI] [PubMed] [Google Scholar]
  22. Taswell C. Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. J Immunol. 1981 Apr;126(4):1614–1619. [PubMed] [Google Scholar]
  23. Weaver L. D., Dellers R. W., Dardiri A. H. Bovine herpes mammillitis in New York. J Am Vet Med Assoc. 1972 Jun 15;160(12):1643–1644. [PubMed] [Google Scholar]
  24. YORK C. J., SCHWARZ A. J., ESTELA L. A. Isolation and identification of infectious bovine rhinotracheitis virus in tissue culture. Proc Soc Exp Biol Med. 1957 Apr;94(4):740–744. doi: 10.3181/00379727-94-23071. [DOI] [PubMed] [Google Scholar]
  25. Yasukawa M., Zarling J. M. Autologous herpes simplex virus-infected cells are lysed by human natural killer cells. J Immunol. 1983 Oct;131(4):2011–2016. [PubMed] [Google Scholar]
  26. Yasukawa M., Zarling J. M. Human cytotoxic T cell clones directed against herpes simplex virus-infected cells. I. Lysis restricted by HLA class II MB and DR antigens. J Immunol. 1984 Jul;133(1):422–427. [PubMed] [Google Scholar]
  27. Yasukawa M., Zarling J. M. Human cytotoxic T cell clones directed against herpes simplex virus-infected cells. II. Bifunctional clones with cytotoxic and virus-induced proliferative activities exhibit herpes simplex virus type 1 and 2 specific or type common reactivities. J Immunol. 1984 Nov;133(5):2736–2742. [PubMed] [Google Scholar]
  28. Yates W. D. A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. Can J Comp Med. 1982 Jul;46(3):225–263. [PMC free article] [PubMed] [Google Scholar]
  29. Zaia J. A., Leary P. L., Levin M. J. Specificity of the blastogenic response of human mononuclear cells to herpesvirus antigens. Infect Immun. 1978 Jun;20(3):646–651. doi: 10.1128/iai.20.3.646-651.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zarling J. M., Moran P. A., Burke R. L., Pachl C., Berman P. W., Lasky L. A. Human cytotoxic T cell clones directed against herpes simplex virus-infected cells. IV. Recognition and activation by cloned glycoproteins gB and gD. J Immunol. 1986 Jun 15;136(12):4669–4673. [PubMed] [Google Scholar]

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