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. 1984 Sep;53(1):79–86.

Inhibition of autologous mixed lymphocyte reaction by monoclonal antibodies specific for the β chain of HLA-DR antigens

M Kasahara, H Ikeda, K Ogasawara, N Ishikawa, T Okuyama, Y Fukasawa, H Kojima, H Kunikane, S Hawkin, T Ohhashi, T Natori, A Wakisaka, Y Kikuchi, M Aizawa
PMCID: PMC1454738  PMID: 6205985

Abstract

Recent studies using rabbit antisera to the separated HLA-DR α and β subunits have suggested that α chain-specific, but not β chain-specific, antisera inhibit T cell proliferative responses in primary and secondary human autologous mixed lymphocyte reaction (AMLR).

In the present study, with the aid of sequential co-precipitation assays and Western blotting methods, a monoclonal rat alloantibody 1E4, specific for the β chain of rat class II molecules carrying an Ia determinant Ba-2.7, was characterized to recognize a monomorphic determinant located on the β chain of DR antigens. This antibody and a murine monoclonal antibody HU-4, also specific for the β chain of DR antigens, strongly inhibited both primary and secondary AMLR through a mechanism distinct from an antibody-dependent cell-mediated cytotoxicity reaction. These results indicate that the inhibition of AMLR is not a unique feature of DR α-specific antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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